See also our related blog for the Pediatric Preclinical Testing Initiative.

Thursday, October 8, 2015

Highlighting Matthew!

our Congratulations to Matthew R. whose review on tissue-specific muscle stem cells has just been published.  You can see the article here.  Matthew wrote this review as part of his work with his PhD mentor, Dr. Grace Pavlath.  Matthew's scientific publication from this training is here.  

Sunday, July 5, 2015

CURE Childhood Cancer Foundation Awards Multiyear Grant to cc-TDI

We are pleased to announce that the Children's Cancer Therapy Development Institute was selected to receive a multiyear research grant awarded by CURE Childhood Cancer foundation of Atlanta, GA.  The grant will help fund our CUREfast Cancer Registry for Familial and Sporadic Tumors.  We have pioneered an approach to the difficult decision that some families make when choosing to donate tissue that provides resources to the scientific community to bring about new knowledge and potential new drug treatments for changed outcomes.  We are excited to work with CURE Childhood Cancer and honored to be selected as one of their grantees.  
  
  

Our thanks to the Shenandoah Sharks!

We are grateful to The Shenandoah Sharks, a Summer swim team in Shenandoah, Texas.  These swimmers and their families recently held a swim-a-thon in memory of Thomas, the brother to a member of the team, Emily.  Thomas had an inconquerable spirit during his battle with childhood cancer.  Fittingly, the motto for this event was Fortitudo: strength-courage-perseverance.  Our thanks go to all the swimmers and their families who made this fundraiser possible, and for remembering Thomas in this way. 
  
[ pictured: Charles holding the Sharks' t-shirt with Thomas' parents, Beni and Cynthia. ]

Welcoming Cora & Adelae

We are excited to welcome our Summer students to the lab this week.
  
Adelae is an incoming freshman at Bates College.  Her project is in our 'skunkworks' of trying to redefine how targeted therapies can be delivered -- by surgeons!  This project involves a lot of 3D printing and robots... the type of experience ideal for Adelae's interest in systems biology and neuroscience.  
  
Cora is a Chemical Engineering student at University of Massachusetts, entering her Junior year this Fall.  Her work will be focused on The Alexa Project, which will define the resources available for drug discovery in NF1-associated Juvenile Pilocytic Astrocytoma (a form of brain tumor).  
    
[ pictured:  Adelae (left) and Cora (right) ] 

Matt, our newest muscle stem cell biologist

We are excited to welcome Matt to our cc-TDI scientific team.  Dr. Matthew Randolph earned his Doctorate of Veterinary Medicine in 2000 from the University of Georgia College of Veterinary Medicine. He practiced mixed animal medicine as an associate veterinarian in northeast Georgia for five years. Matthew then pursued a career in cancer research and recently trained as a stem cell biologist at Emory University, in the laboratory of Dr. Grace Pavlath, while earning his PhD in Biochemistry, Cell, and Developmental Biology.  He has joined cc-TDI as a post-doctoral researcher and will investigate novel therapies for childhood cancers, including the cancer of muscle, rhabdomyosarcoma. Matthew and his wife, Casey, enjoy hiking, working with children, and watching science-fiction shows.

Tuesday, May 5, 2015

Scientists from 13 institutions worldwide collaborate to discover promising therapy for DIPG


Unprecedented international coalition led to breakthrough for patients with deadliest form of childhood brain cancer, as released today in Nature Medicine.



[press release]  FORT COLLINS, CO, May 4, 2015 – Researchers from an international coalition of 13 institutions have discovered a promising combination drug therapy for the deadly childhood cancer Diffuse Intrinsic Pontine Glioma (DIPG). In a study published today in Nature Medicine co-authored by Michelle Monje, MD, PhD (Stanford University) and Charles Keller, MD (Children’s Cancer Therapy Development Institute), the consortium reports that testing of panobinostat, with or without the histone demethylase inhibitor GSKJ4, may be a promising therapeutic strategy for this deadly pediatric brain tumor. 

DIPG is a rare tumor of the brainstem that occurs almost exclusively in children under 10 years old. It represents one of the most devastating diagnoses among pediatric cancers, with an average survival rate of just nine months. As co-Chairs of the Children’s Oncology Group brain tumor new drugs committee, Dr. Keller and Dr. Maryam Fouladi built and coordinated the group of basic and translational scientists who formed the unprecedented coalition. The project was sponsored by the Lyla Nsouli Foundation, The CureStartsNow, Curesearch and Accelerate Brain Cancer Cures and supported by multiple gifts of tumor tissue from children who did not survive.

“The story of how the consortium started is a fun one,” explained Keller. “In a packed St Louis hotel meeting room a few years ago, our committee put forth ideas for the next DIPG clinical trial.  It became clear that the nation’s best oncologists had lots of ideas, but we all had no data…so we decided to have a “bake-off”. Maryam rallied the pediatric neuro-oncologists, and I coordinated and recruited the research teams. Eighty-three drugs later, we have an answer.”

The work began with a chemical screen in patient-derived DIPG cultures along with RNAseq analyses and integrated computational modeling to identify potentially effective therapeutic strategies. The multi-histone deacetylase inhibitor panobinostat demonstrated efficacy in vitro and in DIPG orthotopic xenograft (mouse) models. Further testing of panobinostat with histone demethylase inhibitor GSKJ4 revealed synergy in combination. A clinical trial is currently being designed for single-drug therapy with panobinostat, which will likely begin enrolling patients later this year.

“This panobinostat clinical trial is really the first step,” Keller said. “We can be fairly certain that as the ‘world’s most difficult cancer to treat,’ DIPG will take more than one drug to cure. As promising as the panobinostat-GSKJ4 combination is, we may need to go further—even exploring non-traditional compounds not yet used as medicines.”

The full consortium includes:

Charles Keller, MD - Children's Cancer Therapy Development Institute (co-corresponding author)

Michelle Monje, MD, PhD - Stanford University (co-corresponding author)

Marta Alonso, MD - University of Navarra, Madrid, Spain
Oren Becher, MD - Duke University Medical Center

Mariko DeWire, MD - Nationwide Children's Hospital

Maryam Fouladi, MD - Cincinnati Children's Hospital Medical Center

Jacques Grill, MD, PhD - Institut Gustave-Roussy, Villejuif, France

Cynthia Hawkins, MD, PhD - University of Toronto

Xiao-Nan Li, MD, PhD - Baylor College of Medicine

Dannis G. van Vuurden, MD, MSc, & Esther Hulleman - VU Cancer Center Amsterdam

Eric H. Raabe, MD - Johns Hopkins University

Dennis Steindler, PhD - University of Florida

Katherine Warren, MD, Paul Meltzer, MD, PhD, and Martha Quezado, MD - National Institutes of Health

“The manuscript by Grasso, Tang, Truffaux et al is an outstanding example of cooperation among dedicated laboratory based scientists and clinicians who have pooled resources and freely shared data to impact the treatment of a rare but fatal brain tumor in children. A special recognition should be given to community-based organizations that provided philanthropic support to enable the study. This model of translational research should be encouraged and fostered to improve outcome for rare human disease,” said Amar Gajjar, MD, Chair of the Central Nervous System Tumor Committee, Children's Oncology Group and neuro-oncologist at St. Jude Children’s Research Hospital.

“In a disease where no therapeutic progress has been made in several decades, we see the culmination of the efforts of an international group of basic and translational investigators who have collaborated together to better understand potential therapeutic targets and conduct preclinical testing of promising agents targeting relevant pathways in this deadly disease. The fact that Drs. Monje & Keller and colleagues demonstrated that the combination of panobinostat and the GSKJ4 demonstrate synergy is very promising. The Children’s Oncology Group was the catalyst for fostering these efforts over the past three years, with funding coming from philanthropic agencies who also banded together to support these efforts. The next step is to develop clinical trials to assess the efficacy of these agents alone and in combination in patients with DIPG. Based on data presented in this paper, the Pediatric Brain Tumor Consortium is moving forward with a new study to test panobinostat in children with newly diagnosed and recurrent DIPGs. I hope that the results generated from these critical preclinical collaborations will lead to the development of novel combination trial strategies to effectively treat children with this deadly tumor,” said Maryam Fouladi, MD, Chair of the Pediatric Brain Tumor Consortium Steering Committee and Medical Director of the Neuro-Oncology Program at Cincinnati Children’s Hospital Medical Center.


"For as long as DIPG has been specifically classified, parents of children diagnosed with this cancer have longed for effective treatments. This represents an exciting and promising new chapter that finally provides hope. Since the time of my daughter Alexis' diagnosis in April 2008, and her passing in January 2011, little has changed…until now. I am beyond hopeful that this now represents that change,” said Jonathan Agin, childhood cancer advocate and cc-TDI General Counsel. Agin lost his daughter Alexis to DIPG when she was two weeks away from her fifth birthday.


"We are very enthusiastic about the work of Dr. Keller, Dr. Monje and the DIPG consortium and are excited by the study findings about panobinostat. This drug offers a new path of study with the potential to create treatment options for DIPG. We look forward to the next steps of research and are hopeful that this progress brings us closer to helping children with DIPG to survive.”
Based in Fort Collins, Colorado, the Children's Cancer Therapy Development Institute is a unique research group that is focused on closing what is known as the "pre-clinical gap" in childhood cancer research. To end the stalemate in curing childhood cancers, cc-TDI is dedicated to translating knowledge from basic scientific advancements into proven, viable treatment options that can be tested in clinical trials,” comments Nadim Nsouli on behalf of the Lyla Nsouli Foundation, the principal sponsor of the international research effort.  Mr. Nsouli, and his wife Simone, founded the Lyla Nsouli Foundation after the passing of their 4 year old daughter, Lyla, as a result of DIPG.  Her mother adds, “We miss Lyla terribly. She was truly our pride and joy.” 


Sandy Smith, an advocate for childhood cancer and a cancer survivor herself offered this perspective: “I was diagnosed with breast cancer on Monday, October 22, 2007. My 6-year-old son Andrew was diagnosed with diffuse intrinsic pontine glioma (DIPG) just a few days later. We both ended up with ports, we both did chemotherapy and radiation, and we both lost our hair; but that’s where the similarities end. My treatment was standard and my prognosis favorable. There was no hope of a cure for Andrew; radiation was palliative. While we pushed through my treatment, my husband and I did our best to savor every moment with our son because we knew those moments were very limited. This publication—the result of selfless collaboration by dedicated scientists from around the world—is a step toward progress for children with DIPG. This is meaningful science making a difference in the lives of children and families like mine.”

“This is a journey and a partnership with families.  One of the most interesting features of the project is that we kept a weekly blog of research activities - which allowed families to follow progress with us (and give feedback). We still need more tissue, better cell lines, and more mouse models. Our 15 institutions are unified by the community support that brought us together and keeps us focused. We’ll continue pursuing better and better treatments until the day
that DIPG is uniformly survivable,” Keller added.

For more on cc-TDI’s efforts to move scientific discoveries to clinical trials for children with cancers like DIPG, visit www.cc-TDI.org.

[ full funding acknowledgements: This research was supported by the Lyla Nsouli Foundation, the Children’s Oncology Group (COG) Central Nervous System Committee, the DIPG Collaborative (The Cure Starts Now Foundation, Reflections of Grace Foundation, Smiles for Sophie Foundation, Cancer-Free Kids Foundation, Carly’s Crusade Foundation, Jeffrey Thomas Hayden Foundation, Soar with Grace Foundation), the Accelerate Brain Cancer Cures Foundation (ABC2), CureSearch for Childhood Cancer, the Team Julian Foundation and the COG Chair’s Grant (5UOCA098543). Additional funding support was provided by US National Institutes of Health grant K08NS070926 (to author M.M.), Alex’s Lemonade Stand Foundation  (to M.M. and Y.T.), the McKenna Claire Foundation, the Connor Johnson Memorial Fund, the Dylan Jewett Memorial Fund, the Elizabeth Stein Memorial Fund, the Dylan Frick Memorial Fund, the Abigail Jensen Memorial Fund, the Zoey Ganesh Memorial Fund, the Wayland Villars DIPG Foundation, the Jennifer Kranz Memorial Fund, Unravel Pediatric Cancer, the Virginia & D.K. Ludwig Fund for Cancer Research, the Price Family Charitable Fund, the Matthew Larson Foundation, the Godfrey Family Fund in Memory of Fiona Penelope, the Child Health Research Institute at Stanford, the Anne T. and Robert M. Bass Endowed Faculty Scholarship in Pediatric Cancer  and Blood Diseases (all to M.M.), Etoile de Martin (to J.G. and N.T.), Fondation  Lemos and Le Défi de Fortunée (to J.G.), the Scott Carter Foundation (to N.E.B.),  the Semmy Foundation (to D.G.v.V. and E.H.), the US Department of Defense  (to X.-N.L.), National Science Foundation grant CCF0953366 (to R.P.), Marie Curie grant IRG270459 (to M.M.A.); the Spanish Ministry of Health grant PI13/0125 (to M.M.A.), and the St. Baldrick’s Foundation and Iron Matt Foundation (both to  E.R. and M.H.-C.). ]


###


About Children’s Cancer Therapy Development Institute
The Children's Cancer Therapy Development Institute was created with one aim: to make childhood cancer universally survivable, regardless of diagnosis. Our mission is to translate scientific discovery into clinical trials by understanding and proving new disease-specific treatment options for children with cancer. Our team, led by Scientific Director Charles Keller, MD, is discovering and proving new disease-specific treatments in our Innosphere lab at the base of the Rocky Mountains.

Contact: Charles Keller, MD
(970) 239-4296  | charles@cc-TDI.org
Jonathan Agin |  (703) 371-0218 



coverage in Lancet Oncology:  http://www.thelancet.com/journals/lanonc/article/PIIS1470-2045%2815%2970230-5/abstract

Friday, April 24, 2015

rhabdomyosarcoma conference at NCI

Our thanks to Javed Khan for hosting the NCI Targeting Rhabdomyosarcoma Workshop this week in Gaithersburg.  Highlights included the interaction of muscle biologists and those who study rhabdomyosarcoma.  


Wednesday, April 22, 2015

AACR 2015



The 2015 Annual AACR in Philadelphia meeting concludes today.  It was a particular honor for Dr. Keller to present with his career mentor, 2007 Nobel laureate Mario Capecchi at the session,  Modeling Human Sarcomas: Recent Advances in Organ Site Research.  On Sunday, Dr. Capecchi received the lifetime career award from the AACR.    

Saturday, March 21, 2015

Mayo Clinic seminar

My thanks to hosts Drs. Aubrey Thompson and Al Copland for the opportunity to present our work at cc-TDI in their seminar series.  It was a very productive visit - with new collaborations formed, and special traction on the Xp11 RCC effort.  

Wednesday, March 11, 2015

Congratulations, Mat!

Mat's manuscript on head & neck pediatric rhabdomyosarcoma was accepted today to Frontiers in Oncology, Pediatric Oncology. Co-authors include lab alumni Jinu and GH.  This work was supported in part by the Ethan Jostad Foundation.  stay tuned for the e-release of the paper!
  
[ 03/19/2015 update:  Mat's article is now published online here ]

Monday, March 2, 2015

Purdue Cancer Center seminar

My thanks to hosts Drs. Shihuan Kuang and Timothy Ratliff for the opportunity to present our work at the Purdue University Center for Cancer Research seminar series on February 26.  Purdue has exciting programs in muscle & non-muscle stem cell biology & physiology and tissue engineering, and cancer biology.  It was a pleasure to meet with these accomplished scientists and build new collaborations.  


Sunday, March 1, 2015

NCI Targeting Rhabdomyosarcoma Workshop

reposted from http://ncifrederick.cancer.gov/events/Rhabdomyosarcoma/ 


NCI Shady Grove
8717 Grovemont Circle
Gaithersburg, MD 20877
Host Institute
Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

Background
Rhabdomyosarcoma (RMS) is the third most common extracranial solid tumor of childhood. Approximately 350 new cases are diagnosed in the United States each year accounting for 3 percent of childhood cancers. RMS is derived from primitive myoblasts and morphologically resembles early stages of prenatal skeletal muscle differentiation. However, a large percentage of RMS tumors occur in locations normally lacking skeletal muscle, with the head and neck, genitourinary tract and retroperitoneum being frequent sites of tumor localization. Development of RMS has been associated with genetic tumor predisposition syndromes including Li-Fraumeni syndrome, neurofibromatosis and Costello syndrome. Childhood RMS is subdivided into two major subtypes, embryonal and alveolar, which have distinct histological features and genetic alterations. Adult RMS is largely a third histological subtype, namely pleomorphic RMS. The alveolar RMS subtype carries a poorer prognosis and is strongly myogenin positive by immunohistochemistry. The embryonal RMS subtype carries a better prognosis and is strongly MYOD1 positive by immunohistochemistry. Treatment for RMS is typically multidisciplinary including surgical resection, chemotherapy and radiation therapy. Relapse-free survival rates with this aggressive treatment regimen approach 70-80% for patients with localized disease. However, the 5-year survival rate for patients with metastatic disease at diagnosis continues to be less than 30%. Improvement in these survival rates is dependent upon identification of RMS-specific molecularly targeted agents.
Recent next-generation sequencing efforts have highlighted several driver mutations in RMS. The drivers for ARMS include the PAX3-FOXO1 and PAX7-FOXO1chimeric transcription factors. In contrast, the drivers for ERMS include mutations in the FGFR4/RAS signaling pathway, loss of heterozygosity at 11p15 (leading to IGF2 overexpression) and chromosome 8 gains. The prognostic significance of these mutations is currently unknown. Of these drivers, only IGF1R has been targeted in clinical trials in RMS, through the use of therapeutic monoclonal antibodies. These agents, including cixutumumab, show limited single agent activity and efficacy is limited by tumor resistance to the targeted agent. Small molecule inhibitors of the RAS-MAP kinase pathway, receptor tyrosine kinases, and reactive oxygen species have been validated and tested in other cancer types. In addition, targeted agents directed against other chimeric transcription factors, such as EWS-FLI1, have been identified. Validation of these targeted agents in RMS is complicated by the lack of a universal pre-clinical animal model of ERMS or ARMS.

The purpose of this meeting is to bring together the leaders in rhabdomyosarcoma basic, genomic, translational and clinical research to share ideas, data, resources and plan for collaborative and synergistic approaches to decipher rhabdomyosarcoma biology and develop novel therapies.

Planning committee
Javed Khan, Fred Barr, Doug Hawkins, Stephen Skapek, Janet Shipley, David Langenau, Charles Keller, Mari Yohe

Funding Sources
Office of Rare Disease (ORDR; http://rarediseases.info.nih.gov/)
Center for Cancer Research (CCR; https://ccr.cancer.gov/)

Monday, February 2, 2015

Hope in the Huffington Post


The Children's Cancer Therapy Development Institute was profiled in the Huffington Post today! 


After President Obama revealed an ambitious plan to support personalized medicine in his 2015 State of the Union address, all eyes turned towards the hope this promise might deliver to families dealing with childhood cancer.

Our General Counsel and resident superdad, Jonathan Agin, profiled how cc-TDI will be part of this promise in his heartfelt, informative column. Read the full piece at HuffPost Impact.

Tuesday, January 20, 2015

Welcoming Matthew (to cc-TDI)


Matthew Svalina joins cc-TDI from Dr. Keller's academic laboratory as a founding member of our scientific team, with an eye on advancing the most promising childhood cancer therapeutics towards clinical trials. Matthew is motivated by the 1 in 5 children diagnosed with cancer for whom survival is still a challenge.

Matthew earned a Bachelor of Science degree in Biological Sciences with a minor in Chemistry from the University of Illinois in 2007. Following completion of his undergraduate degree, Matthew earned a paramedic license through Malcolm X College in Chicago, where he completed his field internship with the Chicago Fire Department on ambulances serving the south side of Chicago. As a member of the Keller lab, Matthew has already co-authored two scientific papers on childhood cancer, with additional studies nearing submission. Under Dr. Keller's mentorship, Matthew leads basic science and preclinical projects in rhabdomyosarcoma and medulloblastoma. Matthew’s career goal is to be a physician-scientist with training and independent research in neuroscience and neuro-oncology.


  

Sunday, January 18, 2015

Postdoctoral Fellowship Available


We are now taking applications for a postdoctoral fellowship studying the tumor microenvironment in the childhood cancer of muscle, rhabdomyosarcoma.  This mentored position will explore at depth the cell-cell communication and signaling pathways responsible for tumor initation and progression. A strong publication record and sense of exploration are requisite.  Candidates with PhD degrees in the biological sciences as well as engineering are invited to apply.  For more information, contact Charles Keller, MD at charles@cc-tdi.org.